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Image Search Results
Journal: Frontiers in Endocrinology
Article Title: The DBC1-HIF-1α-PPAR-γ axis regulates Treg cell differentiation to promote myocardial fibrosis in experimental diabetic cardiomyopathy through the paracrine secretion of Areg
doi: 10.3389/fendo.2026.1780666
Figure Lengend Snippet: The expression of DBC1 is elevated in the hearts of DCM mice, contributing to the progression of myocardial fibrosis. (A, B) Quantitative PCR and immunoblot assays with the heart tissues of Control mice and DCM mice and quantification of DBC1 mRNA and protein levels. Statistical test: unpaired two-tailed Student’s t-test. (C, D) Quantification of DBC1 mRNA and protein levels in the heart tissues of DCM mice treated with vehicle, IL-6, or IL-10. (E-L) DBC1 fl/fl or DBC1-CKO C57BL/6 mice were utilized to generate DCM mouse models accompanied with or without myocardial spot injection of DBC1 adenovirus (Ad.DBC1), followed by the collection of mouse hearts. (E) Quantification of DBC1 mRNA in mouse heart tissues. (F, G) The HW/BW ratio and the mean cross-sectional area of left ventricular myocardium. (H) Representative Masson staining of mouse hearts and quantification of collagen volume fraction (CVF)%. (I) Representative immunohistochemical staining of collagen I and collagen III in mouse hearts. (J) The echocardiographic detection of DCM mice and evaluation of LVFS, LVEF, -dP/dt, and +dP/dt. (K) Quantitative PCR of BNP mRNA in mouse heart tissues. (L) The levels of ROS, SOD, MDA, and GSH-Px in mouse heart tissues. Data are representative of n = 5 mice per group. For histological analysis, N = 3–5 sections per heart were quantified and averaged to obtain a single value per mouse. Statistical test: one-way ANOVA with Bonferroni post-hoc test. ** P < 0.01, *** P < 0.001 vs. Control, None, or DBC1 fl/fl ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. DBC1-CKO.
Article Snippet:
Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control, Two Tailed Test, Injection, Staining, Immunohistochemical staining
Journal: Frontiers in Endocrinology
Article Title: The DBC1-HIF-1α-PPAR-γ axis regulates Treg cell differentiation to promote myocardial fibrosis in experimental diabetic cardiomyopathy through the paracrine secretion of Areg
doi: 10.3389/fendo.2026.1780666
Figure Lengend Snippet: DBC1 induces Treg cell differentiation to ST2 hi Areg hi subsets in the hearts of DCM mice. (A-C) DBC1 fl/fl or DBC-CKO C57BL/6 mice were utilized to generate DCM mouse models accompanied with or without myocardial spot injection of DBC1 adenovirus (Ad.DBC1), followed by the collection of mouse hearts. (A) Flow cytometry for Areg and ST2 expression in cardiac single-cell suspension. (B) The mRNA levels of TNF-α, IL-10, TGF-β, IL-1β, and IL-6 in mouse heart tissues. (C) The mRNA expression of HIF-1α and PPAR-γ in mouse heart tissues. (D-F) Treg cells were isolated from the hearts of DBC1 fl/fl mice (DBC1 fl/fl -Treg), DBC1 fl/fl DCM mice (DCM-Treg), and DBC1-CKO DCM mice (CKO-Treg). The CKO-Treg cells were subjected to transfection with OE-DBC1. (D) Flow cytometry for Areg and ST2 expression in isolated Treg cells. (E) The mRNA levels of TNF-α, IL-10, TGF-β, IL-1β, and IL-6 in isolated Treg cells. (F) The mRNA expression of HIF-1α and PPAR-γ in isolated Treg cells. Statistical test: one-way ANOVA with Bonferroni post-hoc test. ** P < 0.01, *** P < 0.001 vs. DBC1 fl/fl or DBC1 fl/fl -Treg; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. DBC1-CKO or DCM-Treg; Δ P < 0.05, ΔΔ P < 0.01, ΔΔΔ P < 0.001 vs. CKO-Treg.
Article Snippet:
Techniques: Cell Differentiation, Injection, Flow Cytometry, Expressing, Single Cell, Suspension, Isolation, Transfection