wild type c57bl 6 mice Search Results


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Inotiv mice
Mice, supplied by Inotiv, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Syagen Technology Inc c57bl/6j hdac6 −/- mice
C57bl/6j Hdac6 −/ Mice, supplied by Syagen Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GemPharmatech Co Ltd three pairs of heterozygous mice il-13+/− c57/bl6
Three Pairs Of Heterozygous Mice Il 13+/− C57/Bl6, supplied by GemPharmatech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BASF wild-type strain c57bl6/sv129 mice
Wild Type Strain C57bl6/Sv129 Mice, supplied by BASF, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Epigenomics ag brain tissue of c57bl/6 wild-type and app/ps mice
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Biomedizinische NMR Forschungs c57bl6/n wild-type mice
C57bl6/N Wild Type Mice, supplied by Biomedizinische NMR Forschungs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bachem c57bl/6 wild-type mice
C57bl/6 Wild Type Mice, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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86
Sanbio Inc type c57bl 6 mice
Type C57bl 6 Mice, supplied by Sanbio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Mudanjiang Youbo Pharmaceutical Co Ltd mouse c57bl 6j male
Mouse C57bl 6j Male, supplied by Mudanjiang Youbo Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amrep Inc wild type c57bl 6 mice
Wild Type C57bl 6 Mice, supplied by Amrep Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Amrep Inc wild type male c57bl 6 mice
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Vital River Laboratories wild type control c57bl 6 mice
The expression of DBC1 is elevated in the hearts of DCM mice, contributing to the progression of myocardial fibrosis. (A, B) Quantitative PCR and immunoblot assays with the heart tissues of Control mice and DCM mice and quantification of DBC1 mRNA and protein levels. Statistical test: unpaired two-tailed Student’s t-test. (C, D) Quantification of DBC1 mRNA and protein levels in the heart tissues of DCM mice treated with vehicle, IL-6, or IL-10. (E-L) DBC1 fl/fl or <t>DBC1-CKO</t> <t>C57BL/6</t> mice were utilized to generate DCM mouse models accompanied with or without myocardial spot injection of DBC1 adenovirus (Ad.DBC1), followed by the collection of mouse hearts. (E) Quantification of DBC1 mRNA in mouse heart tissues. (F, G) The HW/BW ratio and the mean cross-sectional area of left ventricular myocardium. (H) Representative Masson staining of mouse hearts and quantification of collagen volume fraction (CVF)%. (I) Representative immunohistochemical staining of collagen I and collagen III in mouse hearts. (J) The echocardiographic detection of DCM mice and evaluation of LVFS, LVEF, -dP/dt, and +dP/dt. (K) Quantitative PCR of BNP mRNA in mouse heart tissues. (L) The levels of ROS, SOD, MDA, and GSH-Px in mouse heart tissues. Data are representative of n = 5 mice per group. For histological analysis, N = 3–5 sections per heart were quantified and averaged to obtain a single value per mouse. Statistical test: one-way ANOVA with Bonferroni post-hoc test. ** P < 0.01, *** P < 0.001 vs. Control, None, or DBC1 fl/fl ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. DBC1-CKO.
Wild Type Control C57bl 6 Mice, supplied by Vital River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The expression of DBC1 is elevated in the hearts of DCM mice, contributing to the progression of myocardial fibrosis. (A, B) Quantitative PCR and immunoblot assays with the heart tissues of Control mice and DCM mice and quantification of DBC1 mRNA and protein levels. Statistical test: unpaired two-tailed Student’s t-test. (C, D) Quantification of DBC1 mRNA and protein levels in the heart tissues of DCM mice treated with vehicle, IL-6, or IL-10. (E-L) DBC1 fl/fl or DBC1-CKO C57BL/6 mice were utilized to generate DCM mouse models accompanied with or without myocardial spot injection of DBC1 adenovirus (Ad.DBC1), followed by the collection of mouse hearts. (E) Quantification of DBC1 mRNA in mouse heart tissues. (F, G) The HW/BW ratio and the mean cross-sectional area of left ventricular myocardium. (H) Representative Masson staining of mouse hearts and quantification of collagen volume fraction (CVF)%. (I) Representative immunohistochemical staining of collagen I and collagen III in mouse hearts. (J) The echocardiographic detection of DCM mice and evaluation of LVFS, LVEF, -dP/dt, and +dP/dt. (K) Quantitative PCR of BNP mRNA in mouse heart tissues. (L) The levels of ROS, SOD, MDA, and GSH-Px in mouse heart tissues. Data are representative of n = 5 mice per group. For histological analysis, N = 3–5 sections per heart were quantified and averaged to obtain a single value per mouse. Statistical test: one-way ANOVA with Bonferroni post-hoc test. ** P < 0.01, *** P < 0.001 vs. Control, None, or DBC1 fl/fl ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. DBC1-CKO.

Journal: Frontiers in Endocrinology

Article Title: The DBC1-HIF-1α-PPAR-γ axis regulates Treg cell differentiation to promote myocardial fibrosis in experimental diabetic cardiomyopathy through the paracrine secretion of Areg

doi: 10.3389/fendo.2026.1780666

Figure Lengend Snippet: The expression of DBC1 is elevated in the hearts of DCM mice, contributing to the progression of myocardial fibrosis. (A, B) Quantitative PCR and immunoblot assays with the heart tissues of Control mice and DCM mice and quantification of DBC1 mRNA and protein levels. Statistical test: unpaired two-tailed Student’s t-test. (C, D) Quantification of DBC1 mRNA and protein levels in the heart tissues of DCM mice treated with vehicle, IL-6, or IL-10. (E-L) DBC1 fl/fl or DBC1-CKO C57BL/6 mice were utilized to generate DCM mouse models accompanied with or without myocardial spot injection of DBC1 adenovirus (Ad.DBC1), followed by the collection of mouse hearts. (E) Quantification of DBC1 mRNA in mouse heart tissues. (F, G) The HW/BW ratio and the mean cross-sectional area of left ventricular myocardium. (H) Representative Masson staining of mouse hearts and quantification of collagen volume fraction (CVF)%. (I) Representative immunohistochemical staining of collagen I and collagen III in mouse hearts. (J) The echocardiographic detection of DCM mice and evaluation of LVFS, LVEF, -dP/dt, and +dP/dt. (K) Quantitative PCR of BNP mRNA in mouse heart tissues. (L) The levels of ROS, SOD, MDA, and GSH-Px in mouse heart tissues. Data are representative of n = 5 mice per group. For histological analysis, N = 3–5 sections per heart were quantified and averaged to obtain a single value per mouse. Statistical test: one-way ANOVA with Bonferroni post-hoc test. ** P < 0.01, *** P < 0.001 vs. Control, None, or DBC1 fl/fl ; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. DBC1-CKO.

Article Snippet: Wild-type (Control) C57BL/6 mice, HIF-1α fl/fl mice, PPAR-γ fl/fl mice, and Foxp3 Cre C57BL/6 mice (with Cre recombinase knocked into the Foxp3 locus to achieve Treg-specific gene editing) were purchased from Vital River Laboratories (Beijing, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control, Two Tailed Test, Injection, Staining, Immunohistochemical staining

DBC1 induces Treg cell differentiation to ST2 hi Areg hi subsets in the hearts of DCM mice. (A-C) DBC1 fl/fl or DBC-CKO C57BL/6 mice were utilized to generate DCM mouse models accompanied with or without myocardial spot injection of DBC1 adenovirus (Ad.DBC1), followed by the collection of mouse hearts. (A) Flow cytometry for Areg and ST2 expression in cardiac single-cell suspension. (B) The mRNA levels of TNF-α, IL-10, TGF-β, IL-1β, and IL-6 in mouse heart tissues. (C) The mRNA expression of HIF-1α and PPAR-γ in mouse heart tissues. (D-F) Treg cells were isolated from the hearts of DBC1 fl/fl mice (DBC1 fl/fl -Treg), DBC1 fl/fl DCM mice (DCM-Treg), and DBC1-CKO DCM mice (CKO-Treg). The CKO-Treg cells were subjected to transfection with OE-DBC1. (D) Flow cytometry for Areg and ST2 expression in isolated Treg cells. (E) The mRNA levels of TNF-α, IL-10, TGF-β, IL-1β, and IL-6 in isolated Treg cells. (F) The mRNA expression of HIF-1α and PPAR-γ in isolated Treg cells. Statistical test: one-way ANOVA with Bonferroni post-hoc test. ** P < 0.01, *** P < 0.001 vs. DBC1 fl/fl or DBC1 fl/fl -Treg; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. DBC1-CKO or DCM-Treg; Δ P < 0.05, ΔΔ P < 0.01, ΔΔΔ P < 0.001 vs. CKO-Treg.

Journal: Frontiers in Endocrinology

Article Title: The DBC1-HIF-1α-PPAR-γ axis regulates Treg cell differentiation to promote myocardial fibrosis in experimental diabetic cardiomyopathy through the paracrine secretion of Areg

doi: 10.3389/fendo.2026.1780666

Figure Lengend Snippet: DBC1 induces Treg cell differentiation to ST2 hi Areg hi subsets in the hearts of DCM mice. (A-C) DBC1 fl/fl or DBC-CKO C57BL/6 mice were utilized to generate DCM mouse models accompanied with or without myocardial spot injection of DBC1 adenovirus (Ad.DBC1), followed by the collection of mouse hearts. (A) Flow cytometry for Areg and ST2 expression in cardiac single-cell suspension. (B) The mRNA levels of TNF-α, IL-10, TGF-β, IL-1β, and IL-6 in mouse heart tissues. (C) The mRNA expression of HIF-1α and PPAR-γ in mouse heart tissues. (D-F) Treg cells were isolated from the hearts of DBC1 fl/fl mice (DBC1 fl/fl -Treg), DBC1 fl/fl DCM mice (DCM-Treg), and DBC1-CKO DCM mice (CKO-Treg). The CKO-Treg cells were subjected to transfection with OE-DBC1. (D) Flow cytometry for Areg and ST2 expression in isolated Treg cells. (E) The mRNA levels of TNF-α, IL-10, TGF-β, IL-1β, and IL-6 in isolated Treg cells. (F) The mRNA expression of HIF-1α and PPAR-γ in isolated Treg cells. Statistical test: one-way ANOVA with Bonferroni post-hoc test. ** P < 0.01, *** P < 0.001 vs. DBC1 fl/fl or DBC1 fl/fl -Treg; # P < 0.05, ## P < 0.01, ### P < 0.001 vs. DBC1-CKO or DCM-Treg; Δ P < 0.05, ΔΔ P < 0.01, ΔΔΔ P < 0.001 vs. CKO-Treg.

Article Snippet: Wild-type (Control) C57BL/6 mice, HIF-1α fl/fl mice, PPAR-γ fl/fl mice, and Foxp3 Cre C57BL/6 mice (with Cre recombinase knocked into the Foxp3 locus to achieve Treg-specific gene editing) were purchased from Vital River Laboratories (Beijing, China).

Techniques: Cell Differentiation, Injection, Flow Cytometry, Expressing, Single Cell, Suspension, Isolation, Transfection